免费一级毛片中文-免费尤物视频-免费玉足脚交视频-免费在线观看污网站-免费在线视频一区二区-免费综合激情-免费做受视频-免看一级a

咨詢熱線

15000266580

當前位置:首頁 >產品中心>細胞庫>小鼠腫瘤細胞、癌細胞>CRL-1720F9 小鼠畸胎瘤細胞

F9 小鼠畸胎瘤細胞

簡要描述:CRL-1720 F9 小鼠畸胎瘤細胞,原代細胞|細胞系|細胞株|菌種原代細胞、細胞系。細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻優培養條件!

  • 產品型號:CRL-1720
  • 廠商性質:生產廠家
  • 更新時間:2025-06-28
  • 訪  問  量:2165

產品分類

Product Category

詳細介紹

CRL-1720 F9 小鼠畸胎瘤細胞


ATCC® Number:  CRL-1720™

Designations:  F9

Depositors:   S Strickland

Biosafety Level: 1

Shipped:  frozen

Medium & Serum:  See Propagation

Growth Properties: adherent

Organism: Mus musculus (mouse)

Morphology: epithelial

CRL-1720 F9 小鼠畸胎瘤細胞

Source: Organ: testis

Strain: 129

Disease: embryonal carcinoma; testicular teratoma

Cellular Products: plasminogen activator; laminin; type IV collagen

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Applications: transfection host (Roche FuGENE® Transfection Reagents)

Age:  embryo

Comments: F9 cells can be stimulated to differentiate into parietal endoderm in the presence of retinoic acid and dibutyryl cyclic AMP (cAMP).

Differentiating cells synthesize plasminogen activator, laminin and type IV collagen.

cAMP is active only on cells that have been treated with retinoic acid.

The cells maintain three copies of the beta 1 integrin gene.

Tested and found negative for ectromelia virus (mousepox).

Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing:  Protocol: NOTE: culture vessels must be coated with 0.1% gelatin prior to use.To do so, cover the surface of the vessel with 0.1% gelatin (Difco) in sterile distilled water for 2 hours at 4C, then wash three times with sterile distilled water. Treated flasks and dishes can be stored at room temperature.

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new coated culture vessels.

Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:10 is recommended

Medium Renewal: Every 2 to 3 days

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 1160: Strickland S, et al. Hormonal induction of differentiation in teratocarcinoma stem cells: generation of parietal endoderm by retinoic acid and dibutyryl cAMP. Cell 21: 347-355, 1980. PubMed: 6250719

1161: Strickland S, Mahdavi V. The induction of differentiation in teratocarcinoma stem cells by retinoic acid. Cell 15: 393-403, 1978. PubMed: 214238

23426: Stephens LE, et al. Targeted deletion of beta 1 integrins in F9 embryonal carcinoma cells affects morphological differentiation but not tissue-specific gene expression. J. Cell Biol. 123: 1607-1620, 1993. PubMed: 7504677

26151: Berstine EG, et al. Alkaline phosphatase activity in mouse teratoma. Proc. Natl. Acad. Sci. USA 70: 3899-3903, 1973. PubMed: 4521215

32547: Jang SI, et al. Activator protein 1 activity is involved in the regulation of the cell type-specific expression from the proximal promoter of the human profilaggrin gene. J. Biol. Chem. 271: 24105-24114, 1996. PubMed: 8798649



CRL-1720


















產品咨詢

留言框

  • 產品:

  • 您的單位:

  • 您的姓名:

  • 聯系電話:

  • 常用郵箱:

  • 省份:

  • 詳細地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結果(填寫阿拉伯數字),如:三加四=7
聯系方式

郵箱:[email protected]

地址:上海市虹口區四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術支持:化工儀器網    管理登陸
主站蜘蛛池模板: 色欲精品| 无码潮喷a片无码高潮快三 无码潮喷中文 | 欧美专区亚洲专区 | 黄色电影免费看 | 国产精品无码专区在线播放 | 亚洲高清国产一线 | 久久国产老子精品无码久 | 国产成人精品久久亚 | 国产精品自在线拍国产下 | 免费一级片网站 | 国产av无码专区亚洲aw | 久久99精品一级毛片免费看 | 国产毛片久久久久久国产毛片 | 精品国产免费人成在线观看 | 免费精品日本拍在线不卡 | 久久精品噜噜噜 | 亚洲欧美日韩中文另类不卡 | 91大神在线精品视频一区 | 国产乱码一区二区在线观看免费 | 中文亚洲a| 日韩高跟视频一区二区三区 | 欧美制服二区 | 成年人黄色网址 | 欧美综合色婷婷欧美综合五月 | 日韩免费无码久久久 | 国产日韩在线观看视频 | 91中文字幕在线永久在线观看 | 国产成在线观看免费视频成本人 | 99久久综| 人人揉人人捏人人添 | 无码vr最新无码av专区 | 欧美性猛交xxxx乱大交3 | 国产末成年呦交在线 | 正版高清视频在线观看 | 日韩综合网 | 成人av免费视频在线观看 | 丝袜高潮流白浆潮喷在线播放 | 好看的电视剧免费在线观看 | 成年人com日批软件91 | 久久久国产精品亚洲专区91 | 国产精品扒开腿做爽爽 |