免费一级毛片中文-免费尤物视频-免费玉足脚交视频-免费在线观看污网站-免费在线视频一区二区-免费综合激情-免费做受视频-免看一级a

咨詢熱線

15000266580

當前位置:首頁 >產品中心>細胞庫>人正常細胞>G HA-VSMC人血管平滑肌細胞

人血管平滑肌細胞

簡要描述:CRL-1999 T/G HA-VSMC 人血管平滑肌細胞,
原代細胞|細胞系|細胞株|菌種;細胞庫管理規范,
提供的細胞株背景清楚,提供參考文獻和培養條件!

  • 產品型號:G HA-VSMC
  • 廠商性質:生產廠家
  • 更新時間:2025-06-26
  • 訪  問  量:2425

產品分類

Product Category

詳細介紹

CRL-1999 T/G HA-VSMC 人血管平滑肌細胞

ATCC® Number: CRL-1999™ Price: $264.00

Designations: T/G HA-VSMC

Depositors: RR Gifford

Bioped: frozen

Medium & Serum: See Propagation


Growth Propersafety Level: 1

Shipties: adherent

Organism: Homo sapiens (human)

Morphology: fibroblast


CRL-1999 T/G HA-VSMC 人血管平滑肌細胞

Source: Organ: aorta

Tissue: smooth muscle

Disease: normal

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Applons: tranicatisfection host (technology from amaxa)

Reverse Transcript: N

DNA Profile (STR): Amelogenin: X

CSF1PO: 10,11

D13S317: 11,12

D16S539: 12

D5S818: 12,13

D7S820: 9,10

THO1: 7,8

TPOX: 8

vWA: 16

Cytogenetic Analysis: This is a normal human cell line with a 46,XX karyotype. The modal chromosome number was 46, occurring in 90% of cells. The rate of polyploid cells was 0.8%. No structurally altered chromosomes were detected in 15 metaphases karyotyped. Both X chromosomes appeared normal.

Age: 11 months

Gender: female

Ethnicity: Caucasian

HeLa Markers: N

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: 0.05 mg/ml ascorbic acid; 0.01 mg/ml insulin; 0.01 mg/ml transferrin; 10 ng/ml sodium selenite; 0.03 mg/ml Endothelial Cell Growth Supplement (ECGS); fetal bovine serum to a final concentration of 10%, HEPES to a final concentration of 10 mM, TES to a final concentration of 10 mM.

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution, and incubate at 37C for 5 minutes. Add an equal volume of fresh medium, disperse the cells and centrifuge to pellet the cells. Resuspend the cells in fresh medium, aspirate and dispense into new flasks.

Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:3 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004

recommended serum:ATCC 30-2020



CRL-1999 T/G HA-VSMC





















產品咨詢

留言框

  • 產品:

  • 您的單位:

  • 您的姓名:

  • 聯系電話:

  • 常用郵箱:

  • 省份:

  • 詳細地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結果(填寫阿拉伯數字),如:三加四=7
聯系方式

郵箱:[email protected]

地址:上海市虹口區四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術支持:化工儀器網    管理登陸
主站蜘蛛池模板: 精品三级欧美中文 | 国产乱伦高清影视 | 自拍偷自拍亚洲一区 | 亚洲日本| 国产精品美女久久久久久吹潮 | 永久免费观看美女裸体的网站 | 久久99亚洲精品片片 | 免费国产va在线观看视频 | 伊人久久大香线蕉影院 | 一区二区三区四区国产免费 | 免费黄色视频在线观看 | 中文字幕在线视频第一页 | 欧美午夜片在线观看爱看网 | 亚洲综合国产成人丁香五月激情 | 欧美另类激 | 97人妻精品一区二区三区 | 在线免费观欧美一级片 | 免费高清国产在线视频 | 黄色视频在线无码浏览 | 亚洲乱码一区二区欧美在线观看 | 97无码免费人妻超级碰碰夜夜 | 国产精品久久久久 | 国产亚洲日韩欧美另类第八页 | 午夜欧美精品久久久久久久 | 午夜男女爽爽羞羞影院在线观看 | 亚洲人成网站十八 | 欧美精品亚洲精品日韩已满十八 | 麻豆成人久久精品二区三区色欲 | 国产综合久久精品东京热 | 一本久久a久久精品亚洲 | 日韩高清性爽一级毛片免费 | 国产精品亚洲欧美高清 | 国产啪精品视频网站免费尤 | 午夜片少妇无码区 | 久久精品中文骚妇内射av | 精品日本免费一区二区三区 | 国产的av在线播放 | 日本中文字幕?∨在线观看 日本中文字幕二区三区 | 日本大乳奶电影在线观看 | 国产日韩欧美精品一区二区三区 | 国产最爽的乱淫视频国语对白 |