免费一级毛片中文-免费尤物视频-免费玉足脚交视频-免费在线观看污网站-免费在线视频一区二区-免费综合激情-免费做受视频-免看一级a

咨詢熱線

15000266580

當前位置:首頁 >產(chǎn)品中心>細胞庫>人正常細胞>CRL-11268 293T/17人胚腎細胞

CRL-11268 293T/17人胚腎細胞

簡要描述:CRL-11268 293T/17人胚腎細胞
,ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和培養(yǎng)條件

  • 產(chǎn)品型號:
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2025-07-08
  • 訪  問  量:2069

產(chǎn)品分類

Product Category

詳細介紹

CRL-11268 293T/17人胚腎細胞

Permits and Restrictions

View Restrictions

OrganismHomo sapiens, human
Tissuekidney
Product Formatfrozen
Morphologyepithelial
Culture Propertiesadherent
Biosafety Level2 [Cells contain Adeno and SV-40 viral DNA sequences]
Agefetus
Applications

These cells constitutively express the simian virus 40 (SV40) large T antigen, and clone 17 was selected specifically for its high transfectability.  CRL-11268 293T/17人胚腎細胞

Storage Conditionsliquid nitrogen vapor phase
Derivation

The 293T/17 cell line is a derivative of the 293T (293tsA1609neo) cell line. 293T is a hmperature sensitive gene for SV40 T-antigen was inserted. 293T cells were cloned anighly transfectable derivative of the 293 cell line into which the ted the clones tested with the pBND and pZAP vectors to obtain a line capable of producing high titers of infectious retrovirus, 293T/17.

Antigen Expression

SV40 T antigen

Genes Expressed

SV40 T antigen

Comments

293T/17 cells were cotransfected with the pCRIPenv- and the pCRIPgag-2 vectors to obtain the ANJOU 65 (see ATCC CRL-11269) cell line. ANJOU 65 cells were cotransfected with the pCRIPgag-2 and pGPT2E vectors to obtain the BOSC 23 (see ATCC CRL-11270) ecotropic envelope-expression packaging cell line. ANJOU 65 cells were also cotransfected with the pCRIPAMgag vector along with a plasmid expressing the gpt resistance gene to obtain the Bing (see ATCC CRL-11554)amphotropic envelope-expression packaging cell line.

Complete Growth MediumThe base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.      
SubculturingVolumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.

S

Medium Renewal: Every 2 to 3 days

Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯數(shù)字),如:三加四=7
聯(lián)系方式

郵箱:[email protected]

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
主站蜘蛛池模板: 亚洲一日欧美日韩中文字幕 | 欧美日韩在线播放成人 | 久久亚洲私人国产精品 | 欧美一级性爱视屏 | 91久久国产视频 | 欧美成人国产在线观看 | 97尤物人妻在线视频 | 欧美成人一区二区三区在线观看 | 在线观看日 | 女人的天堂a国产 | 国产成人精品无码免费播放 | 午夜dy888国 午夜不卡影 | 在线影院中文字幕 | 亚洲毛片αv无 | 亚洲a电影 | 日韩午夜中文字幕电影 | 欧美日韩第一页中文字幕 | 亚洲中文乱码 | 国产精品自产拍高潮在 | 久久精品国产清高在天天线 | 国产日本一区二区三区 | 国产大爆乳大爆乳在线播放 | 国产一区二区日本在线观看 | 国产成人精品无码免费播放 | 日本中文字幕视频一区二区三区 | 欧美日韩亚洲国产综合 | 欧美黑人巨大xxxxx | 日韩无码动漫精品中文字幕 | 日韩av无码伊人 | 亚洲国产综合精品中文一区二区 | 亚洲精品美女在线观看 | 久久天天躁狠狠躁夜 | 国产午夜免费一区二区三区 | 日韩av无码专区国产乱码网站 | 国产精品污网站午夜www | 一级黄色网站在线免费 | 午夜天天福利小视频 | 在线无码福利看片 | 乱伦日本影视 | 久久青草国产免费观看 | 无码福利一区二区三区 |